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(A) Tumor curve following treatment of Tscm cells with various vaccine conjugates (n=7). Statistics accessed by two-way ANOVA. (B) Survival curve following treatment of Tscm cells with various vaccine conjugates (n=35). Statistics accessed by log-rank test. (C) Bar graphs showing the frequency of CD45.2 + transferred cells in the tumor, tdLN, and spleen by treatment group (n=3). Statistics accessed by ANOVA. (D) Stacked bar graphs indicating the percentages of PD1 and TCF1 subpopulations in tumor, tdLN, and spleen (n=3). Statistics assessed by ANOVA. (E) Bar graph showing the serum cytokine levels (pg/mL) <t>of</t> <t>IFN-α</t> 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (F) Bar graph showing the serum cytokine levels (pg/mL) <t>of</t> <t>IFN-β</t> 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (G) Bar graph showing the serum cytokine levels (pg/mL) of IP-10 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (H) Heatmap of Z-scored serum cytokine data taken 6 hours post-vaccination in indicated treatment groups (n=5). (I) Bar graph showing the serum cytokine levels (pg/mL) of IFN-γ 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (J) Bar graph showing frequency by flow cytometry of IFN-γ + cells among live, CD8 + T cells in the spleen three days following vaccination (n=3). Statistics assessed by ANOVA.
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(A) Tumor curve following treatment of Tscm cells with various vaccine conjugates (n=7). Statistics accessed by two-way ANOVA. (B) Survival curve following treatment of Tscm cells with various vaccine conjugates (n=35). Statistics accessed by log-rank test. (C) Bar graphs showing the frequency of CD45.2 + transferred cells in the tumor, tdLN, and spleen by treatment group (n=3). Statistics accessed by ANOVA. (D) Stacked bar graphs indicating the percentages of PD1 and TCF1 subpopulations in tumor, tdLN, and spleen (n=3). Statistics assessed by ANOVA. (E) Bar graph showing the serum cytokine levels (pg/mL) of IFN-α 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (F) Bar graph showing the serum cytokine levels (pg/mL) of IFN-β 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (G) Bar graph showing the serum cytokine levels (pg/mL) of IP-10 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (H) Heatmap of Z-scored serum cytokine data taken 6 hours post-vaccination in indicated treatment groups (n=5). (I) Bar graph showing the serum cytokine levels (pg/mL) of IFN-γ 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (J) Bar graph showing frequency by flow cytometry of IFN-γ + cells among live, CD8 + T cells in the spleen three days following vaccination (n=3). Statistics assessed by ANOVA.

Journal: bioRxiv

Article Title: Spatiotemporal dynamics of adoptively transferred stem-like CD8 + T cells in the tumor microenvironment following vaccination

doi: 10.64898/2026.05.12.724323

Figure Lengend Snippet: (A) Tumor curve following treatment of Tscm cells with various vaccine conjugates (n=7). Statistics accessed by two-way ANOVA. (B) Survival curve following treatment of Tscm cells with various vaccine conjugates (n=35). Statistics accessed by log-rank test. (C) Bar graphs showing the frequency of CD45.2 + transferred cells in the tumor, tdLN, and spleen by treatment group (n=3). Statistics accessed by ANOVA. (D) Stacked bar graphs indicating the percentages of PD1 and TCF1 subpopulations in tumor, tdLN, and spleen (n=3). Statistics assessed by ANOVA. (E) Bar graph showing the serum cytokine levels (pg/mL) of IFN-α 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (F) Bar graph showing the serum cytokine levels (pg/mL) of IFN-β 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (G) Bar graph showing the serum cytokine levels (pg/mL) of IP-10 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (H) Heatmap of Z-scored serum cytokine data taken 6 hours post-vaccination in indicated treatment groups (n=5). (I) Bar graph showing the serum cytokine levels (pg/mL) of IFN-γ 6 hours post-vaccination in indicated treatment groups (n=5). Statistics assessed by ANOVA. (J) Bar graph showing frequency by flow cytometry of IFN-γ + cells among live, CD8 + T cells in the spleen three days following vaccination (n=3). Statistics assessed by ANOVA.

Article Snippet: ELISA kits were used to measure IFN-α and IFN-β (PBL Assay Science) and ran in accordance with manufacturer’s instructions.

Techniques: Flow Cytometry